Coordinated dispatching of proximity sensors for the surveillance of manoeuvring targets

The surveillance of a manoeuvring target with multiple sensors in a coordinated manner requires a method for selecting and positioning groups of sensors in real time. Herein, the principles of dispatching, as used for the effective operation of service vehicles, are considered. The object trajectory is first discretized into a number of demand instants (data acquisition times), to which groups of sensors are assigned, respectively. Heuristic rules are used to determine the composition of each sensor group by evaluating the potential contribution of each sensor. In the case of dynamic sensors, the position of each sensor with respect to the target is also specified. Our proposed approach aims to improve the quality of the surveillance data in three ways: (1) The assigned sensors are manoeuvred into “optimal” sensing positions, (2) the uncertainty of the measured data is mitigated through sensor fusion, and (3) the poses of the unassigned sensors are adjusted to ensure that the surveillance system can react to future object manoeuvres. If a priori target trajectory information is available, the system performance may be further improved by optimizing the initial pose of each sensor off-line. The advantages of dispatching dynamic sensors over similar static-sensor systems are demonstrated through comprehensive simulations.     

Sensitivity of CFP/YFP and GFP/mCherry pairs to donor photobleaching on FRET determination by fluorescence lifetime imaging microscopy in living cells

Fluorescent protein-based FRET is a powerful method for visualizing protein-protein interactions and biochemical reactions in living cells. It can be difficult, however, to avoid photobleaching when observing fluorescent cells under the microscope, especially those expressing CFP. We compared the sensitivity of two protein-based FRET pairs to light-induced fluorescence changes in the donor, on FRET determination by fluorescence lifetime imaging microscopy (FLIM). Thanks to the very low excitation light levels of the time- and space-correlated single photon counting (TSCSPC) method, FLIM acquisitions were achieved without donor photobleaching. Here, we show that photobleaching of CFP by a mercury lamp under the microscope induced a decrease in the mean fluorescence lifetime, which interfered with FRET determination between CFP and YFP. Importantly, the range of light-induced variation of the mean fluorescence lifetime of CFP was not proportional to the decrease in the steady state fluorescence intensity and varied from cell to cell. The choice of the CFP/YFP pair therefore requires that the cells be observed and analyzed at very low light levels during the whole FRET experiment. In contrast, the GFP/mCherry pair provided an accurate FRET measurement by FLIM, even if some GFP photobleaching took place. We thus demonstrate that CFP can be an unreliable donor for FRET determination in living cells, due to its photosensitivity properties. We demonstrate that the GFP/mCherry pair is better suited for FRET measurement by FLIM in living cells than the CFP/YFP pair.     

Biosorption of Acid Red P-2BX by lichens as low-cost biosorbents

The aim of this study is to examine the dye biosorption properties of lichen species called Cladonia convoluta and Evernia prunastri. Since lichens are extensively found in the environment, their suitability as a cheap adsorbent has been investigated in this study. The optimal parameters for textile dye biosorption were also determined. The dried lichen biomass showed better dye biosorption capacity than ash lichen biomass. C. convoluta had better dye biosorption capacity than E. prunastri. Dye biosorption rate was found as 71.41% at optimal conditions. This study concluded that C. convoluta was a successful and cheap biosorbent for treatment of water contaminated by Acid Red P-2BX dye.     

Mechanical properties of submicronic and nanometric boron carbides obtained by Spark Plasma Sintering: Influence of B/C ratio and oxygen content

Boron carbide samples exhibiting nanometric and submicronic microstructure were sintered by Spark Plasma Sintering to investigate the effect of grain size on mechanical properties. The mechanical properties of sintered monoliths were characterized at the grain and macroscopic scales. Although nanostructured material exhibits finer grains than the submicronic material (i.e. mean diameter of 82 vs. 474?nm), its apparent rigidity and hardness are found to be reduced by 6.8% and 8.4% respectively. This contradiction with the Hall-Petch law is linked to the chemical compositions of both materials, which show significant difference in terms of B/C ratio and higher structural oxygen content especially for nanostructured material.     

Mechanical properties of freely suspended semiconducting graphene-like layers based on MoS2

We fabricate freely suspended nanosheets of molybdenum disulphide (MoS₂) which are characterized by quantitative optical microscopy and high-resolution friction force microscopy. We study the elastic deformation of freely suspended nanosheets of MoS₂ using an atomic force microscope. The Young''s modulus and the initial pre-tension of the nanosheets are determined by performing a nanoscopic version of a bending test experiment. MoS₂ sheets show high elasticity and an extremely high Young''s modulus (0.30 TPa, 50% larger than steel). These results make them a potential alternative to graphene in applications requiring flexible semiconductor materials.PACS, 73.61.Le, other inorganic semiconductors, 68.65.Ac, multilayers, 62.20.de, elastic moduli, 81.40.Jj, elasticity and anelasticity, stress-strain relations.     

Numerical method for impulse control of piecewise deterministic Markov processes

This paper presents a numerical method to calculate the value function for a general discounted impulse control problem for piecewise deterministic Markov processes. Our approach is based on a quantization technique for the underlying Markov chain defined by the post jump location and inter-arrival time. Convergence results are obtained and more importantly we are able to give a convergence rate of the algorithm. The paper is illustrated by a numerical example.     

Cyclic distillation technology – a mini‐review

Process intensification in distillation systems has received much attention during past decades, with the aim of increasing both energy and separation efficiency. Various techniques, such as internal heat‐integrated distillation, membrane distillation, rotating packed bed, dividing‐wall columns and reactive distillation were studied and reported in the literature. All these techniques employ the conventional continuous counter‐current contact of vapor and liquid phases. Cyclic distillation technology is based on an alternative operating mode using separate phase movement which leads to key practical advantages in both chemical and biochemical processes. This article provides a mini‐review of cyclic distillation technology. The topics covered include the working principle, design and control methods, main benefits and limitations as well as current industrial applications. Cyclic distillation can be rather easily implemented in existing columns by simply changing the internals and the operating mode, thus bringing new life to old distillation towers by significantly increasing the column throughput, reducing the energy requirements and offering better separation performance. © 2016 Society of Chemical Industry     

Change detection in coral reef communities using Ikonos satellite sensor imagery and historic aerial photographs

For decades, aerial photographs have been the only source of very high spatial resolution data for coral reef researchers. With the launch of the Ikonos satellite in 1999, imagery with a 4 m spatial resolution in multispectral mode can now be combined with historical aerial photographs for change detection. We demonstrate this potential by combining two aerial photographs (1981 and 1992) and an Ikonos image (2000) to detect change in the coral reef communities for Carysfort Reef, Florida, USA. The results show a loss of 'coral-dominated' bottom from 52% (1981) to 16% (1992) to finally 6% (2000), a trend similar to in situ observations.